As our ablation approach is refractory to population-based analyses like western blotting to assess the abundance of these fragments and it has been established that chlamydial infection induces apoptosis in neighbouring uninfected cells, we applied correlative live cell and immunofluorescence microscopy with a cleaved caspase-3 specific antibody to determine whether or not inclusion rupture leads to activation of endogenous caspase-3 (Supplementary Fig. 3a,b, Supplementary Movie 15). This evidence concerns the gene CASP3 and chlamydia trachomatis infectious disease.