RAB25 and infection: A HeLa reporter cell line stably expressing mCherry-tagged Rab25 (to monitor the integrity of chlamydial inclusions throughout the infection)17, CFP-Histone 2-B (to highlight the nuclei) and soluble green fluorescent protein (GFP) (to mark the cytoplasm) was infected with C. trachomatis strain LGVII (CTL2) at an multiplicity of infection (MOI)∼0.5 and examined by time-lapse videomicroscopy.