In particular, when investigated in biopsy material, up to 60% of G1/early HCC shows immunoreactivity to GPC3, either as membrane and/or cytoplasmic staining, as well as 8% of high-grade DN (4) and occasional cirrhotic fragments (5); figures to be compared to the 85% staining observed in G2–G3 HCC. This evidence concerns the gene GPC3 and hepatocellular carcinoma.