MCAM and melanoma: Furthermore, they suggest a particularly accurate design of primers and probes in order to minimize the effect of endothelial contamination.136 According to this last consideration, we describe our preliminary pilot study performed on CMCs isolated and enriched from patients affected by melanoma at least by AJCC stage Ib, by targeting the MCAM/MUC18/CD146 antigen with immune-magnetic beads coated with antibody against MCAM/MUC18/CD146 antigen.