Furthermore, the immunehistological analysis showed that the elevated active caspase3 and reduced PCNA were detected in the xenografts from RCC CSCs treated with MRK-003 or transfected with Numb (Fig. 5b and c), indicating inhibition of notch1/2 increased apoptosis and decreased proliferation of RCC CSCs in vivo. The gene discussed is NUMB; the disease is renal cell carcinoma.