Our results further confirmed that CD133+/CD24+ tumor cells isolated from RCC ACHN or Caki-1 cell line cells expressed by higher level of stemness marker and possessed self-renewal ability validated by soft agar colony formation and spheres formation assays, resistance to cisplatin and sorafenib, stronger ability to form tumor in vivo, and higher invasive and migratory potential validated bytranswell assay. The gene discussed is CD24; the disease is renal cell carcinoma.