CHUK and melanoma: In order to assess the role of the NF-κB pathway activation in the survival of the melanoma cells treated with PDT, western blot was performed to quantify the protein levels of the total NF-κB protein and the active form, pNF-κB—p65 (at Ser 536) and also the IκKα, IκKβ and the phosphoryted pIκK.