Colony-formation assays indicated that clonogenic survival was significantly decreased in si-BLACAT1-transfected HCT116 and SW480 cells (Figure 2b), Similarly, the results of MTT assays revealed that cell growth was inhibited following downregulation of BLACAT1 in si-BLACAT1-transfected CRC cells compared with respective controls (Figure 2c). The gene discussed is BLACAT1; the disease is colorectal carcinoma.