These predictions can be taken as evidence of L-arginine-NO pathway regulation by miRNAs during infection with La-WT L. (L.)amazonensis and are corroborated by the data obtained during La-arg− infection, such as the increased Nos2 mRNA expression (Fig. 2A), NOS2 (Fig. 3A) and NO production (Fig. 3B and C) after 4 h of infection. The gene discussed is NOS2; the disease is infection.