The miScript Target Protector (Qiagen) competition assay for the binding site of miR-294 to the Nos2 3′UTR using 0.1 μM or 0.5 μM miR-294/Nos2 revealed a significant increase (20% to 22%) in the levels of NOS2 after 4 h of infection (Fig. 6A). The gene discussed is NOS2; the disease is infection.