The reduction in miR-294 expression did not promote a statistically significant change in the amount of Nos2 mRNA (Fig. 5B), even so NOS2 expression (Fig. 5C) and NO production was increased after 4 and 24 h of infection (Fig. 5D), parallel to a 25–30% decrease in infectivity (Fig. 5E). This evidence concerns the gene NOS2 and infection.