AFP and hepatocellular carcinoma: In the current cohort of HCC patients within MC, the ROC analysis indicated that the serum AFP level ≤321 ng/mL (AFP cut-off of >321 ng/mL was associated with approximately a 3-fold increased risk of HCC recurrence after LT) was the optimal cut-off value for HCC recurrence prediction, which was close to the value of the Hangzhou standard (400 ng/mL).