Relationship of PD-L1 expression with response to nCRT is not consistent among studies, possibly due to some technical and biological issues, including inappropriate tumor sampling, variable PD-L1 primary antibodies, different thresholds for PD-L1 positivity, variability of tissue preparation and processing, dynamic and heterogeneous PD-L1 expression, and PD-L1 expression in tumor cells versus infiltrating immune cells [80,81]. Here, CD274 is linked to neoplasm.