Nikas et al. indicated in 108 rectal cancer patients that homozygous C/C genotype in MTHFR gene (rs1801133) were 2.91 times more likely to respond to nCRT (p = 0.015) and 3.25 times more likely not to have recurrence (p = 0.008) than either the heterozygous C/T or homozygous T/T genotype [132], which is consistent with previous study [133]. Here, MTHFR is linked to rectal cancer.