In the present study, the ameliorating effect of DL0410 on the cognitive impairment induced by scopolamine was evaluated, and functions of the cholinergic system, including the activity of AChE and BuChE and ACh levels, and the binding mode of DL0410 in the active site of AChE, were also characterized to further illustrate its potential mechanism. Here, ACHE is linked to Cognitive impairment.