In order to explore the role of AKT2 in cardiac apoptosis during ischemia, cultured NRCMs were treated with AKT2 inhibitor for 3 h before hypoxia treatment, afterwards experimental ischemia (hereafter, ischemia) was achieved by culturing cells inside a hypoxic incubator in Tyrode’s solution without serum and glucose. The gene discussed is AKT2; the disease is ischemia.