Fifth, by developing a VCAM-1-D6 blocking antibody, VCAM-1-D6 huMab, we demonstrated that antibody-based inhibition of VCAM-1-D6 effectively reduced VCAM-1-mediated migration of A549 and NCI-H1299 lung cancer cell lines into Matrigel. This evidence concerns the gene VCAM1 and lung carcinoma.