Given that miR-US5-1 and miR-UL112-3p play a role in modulating the NF-κB response through targeting IKKα and IKKβ, we asked whether NF-κB signaling initiated by viral infection is altered in cells infected with the miR-US5-1/miR-UL112-3p double mutant virus. The gene discussed is CHUK; the disease is viral infectious disease.