CHUK and infection: In order to determine the functional role for HCMV miRNA targeting of IKKα and IKKβ during infection, we derived HCMVs that carry mutations in miR-UL112-3p that disrupt miRNA expression while maintaining the open reading frame sequence of UL114 (37) and in which the miR-US5-1 pre-miRNA sequence was removed (42).