Using a univariate analysis of the correlation between positive surgical margins and clinicopathologic features, the unfavorable tumor regression (TRG 3–4 versus TRG 0–1; p = 0.030), CEA level > 5 (p = 0.041), and ypT4 disease (p = 0.042) were associated with positive surgical margins. The gene discussed is CEACAM5; the disease is neoplasm.