To investigate whether miR-124 inhibited CCA invasion and metastasis through targeting GATA6, plasmids carrying the entire coding sequence of GATA6 without the miR-124 binding sites were transfected into QBC939 cells to rescue miR-124 -induced downregulation of GATA6 (Fig. 4a and b). Here, GATA6 is linked to cholangiocarcinoma.