We updated the meta-analysis of the MTHFR (677C > T (rs1801133) and 1298A > C (rs1801131)), ABCB1 3435C > T (rs1045642), RFC-1 80G > A (rs1051266), and ATIC 347C > G (rs2372536) polymorphisms, completed the first meta-analysis on the association between SLC19A1 G > A (rs7499), SLC19A1 A > G (rs2838956), TYMS 28 bp VNTR (rs34743033) and MTRR 66A > G (rs1801394) polymorphisms and the effectiveness of MTX in RA patients. Here, TYMS is linked to rheumatoid arthritis.