A targeted and integrative serum metabolomics approach (Bachmayr-Heyda & Aust et al. 20) with PD-L1 tumor cell expression in 19 primary and 17 metastatic tumor tissues of 22 patients as outcome revealed 65 out of 182 serum metabolites as significantly (FDR 5%) associated with PD-L1 abundance, thereof 56 glycerophosphilipids, four sphingolipids, three amino acids (his, trp, and tyr; the former two essential proteinogenic amino acids), and two biogenic amines (ADMA and putrescine). The gene discussed is CD274; the disease is metastatic neoplasm.