ENO1 and breast cancer: By using a targeted approach employing MIDAS, a SRM assay was successfully designed based on a limited set of in silico predicted transitions in a study aimed at identifying protein level changes in MCF-7 cells in response to temporal IGF-1R stimulation, where ENO1 showed up at higher levels in breast cancer cell versus their normal counterparts; ENO1, PKM2, and LDHA were also upregulated in both invasive MDA-MB-231 cells and in IGF-1-induced MCF-7 cell, which indicated IGF-1 involvement in tumor invasiveness (116).