MBD2 and cervical carcinoma: A global decrease in methylation was observed for HeLa cervical carcinoma cells overexpressing the WT demethylase as determined by reduction in the levels of methyl-lysine antibody staining (e.g. KDM5B overexpression correlating with H3K4me3 nuclear staining in Fig. 2a iv–vi), relative to cells overexpressing the corresponding catalytically inactive MUT demethylase or nontransfected cells (Fig. 2a vii–ix).