TNF and diabetes mellitus: As shown in Figures 2(a), 2(b), and 2(c), plasma IL-6 and TNF-α levels were increased in the rats with diabetes along with significant increase of 15-2t-IsoP (all p < 0.05 D4w versus nondiabetic group), and these were all further exacerbated by myocardial I/RI (p < 0.05, D4w + I/R versus D4w).