To further investigated the role of ITGA1 in HCC, we selected the HCC cell lines MHCC-97L, MHCC-LM3, and HCC-LY10, which show intrinsically lower expression of ITGA1, to reconstruct ITGA1 stably overexpression in HCC cells by using ITGA1 lentiviral infection and control lentivirus EX-NEG, also known as E6. The gene discussed is ITGA1; the disease is hepatocellular carcinoma.