Combined with the fold changes and biological function of the differentially expressed genes, we finally targeted against ITGA1 and confirmed the downregulation of ITGA1 in HCC cell lines Huh7, SMMC-7721, MHCC-97L, and MHCC-LM3 via qRT-PCR and western blotting analyses (Figure 1C). The gene discussed is ITGA1; the disease is hepatocellular carcinoma.