The results indicated that EMT was inhibited in HepG2-Runx2 cells after LGALS3 knockdown, and EMT was stimulated in Galectin-3 upregulated SMMC7721-shRunx2 cells, which suggested that Runx2 might regulate EMT in HCC cells via Galectin-3, which functioned intermediately in HCC cells. This evidence concerns the gene LGALS3 and hepatocellular carcinoma.