HDAC6 and Miyoshi myopathy: To assess relative selectivity of this compound in MM cells, MM.1s cells were incubated with increasing concentrations of ACY-241 followed by assessment of lysine-40 acetylation (Ac) of α-Tubulin (an HDAC6 specific substrate) [30] and lysine-9 acetylation of Histone-H3 (H3K9; a Class I HDAC specific substrate) [31].