We also found in a 2D screen of 51 mAbs, including AIIB2, against cell surface-associated molecules using the melanoma cell line HTB-66 as well as fresh melanoma, that the anti-CD44 mAb H4C4 blocks coalescence, and this observation was repeated in 4D reconstructions in the 3D Matrigel model. This evidence concerns the gene CD44 and melanoma.