Immunostaining, using antibodies to detect cleaved caspase 3 demonstrated increased apoptosis in an TRE HA-MEKGF+Tet-off tumor collected following 96 h of Dox treatment compared to an untreated TRE HA-MEKGF+Tet-off tumor (Supplementary Figure 7). The gene discussed is CASP3; the disease is neoplasm.