BRAF and melanoma: To assess resistance to BRAF, MEK and ERK inhibition, three well-characterized human BRAFV600E melanoma cell lines (A375, M14 and SKMEL5) were infected with bicistronic GFP lentivirus containing either wild-type MEK, MEKV60E, MEKC121S, MEKG128V, MEKP124L or MEKV154L while they were maintained in media containing a BRAF inhibitor (vemurafenib or dabrafenib).