SMPD3 and neoplasm: As expected, mice with both nSMase2 KD cell lines had a significant reduction in metastasis in the peritoneal cavity compared with nSMase2-negative control cells (for ES-2 and A2780 cells, mice were killed 2 and 4 weeks after injection, respectively), but there was no difference in the primary tumour size (Fig. 2d and Supplementary Fig. 3b).