In order to confirm that miR-139-5p, a noncoding RNA, regulates EMT-related genes (ZEB1, ZEB2, E-cadherin), we overexpressed miR-139-5p by transiently transfecting CRC cell lines with miR-139-5p mimics or scrambled controls, and evaluated the protein expression of these downstream target genes. This evidence concerns the gene ZEB2 and colorectal carcinoma.