To confirm that BCL-RAMBO or BNIP3 do not contribute to L. pneumophila infection in vivo, bacterial burdens were calculated from the lungs of WT C57BL/6, BCL-RAMBO−/−, and BNIP3−/− mice 48 h after infection with ΔflaA L. pneumophila. This evidence concerns the gene BNIP3 and infection.