The data presented here, using In vitro and in vivo models, suggest that activation of MMP-2 and MMP-9 is involved in renal injury induced by Loxosceles venom and its main toxin SMases D and that the use of tetracycline may have a protective effect on Loxosceles venom and SMase D induced kidney damage and could potentially aid the treatment of systemic loxoscelism. The gene discussed is MMP9; the disease is Nephropathy.