HIF1A and melanoma: The results, shown in Figure 3C, demonstrate that vanillin strongly attenuates HIF-1α accumulation increased by MG132 (which blocks all proteolytic activity of the 26S proteasome complex) after blocking the de novo synthesis of HIF-1α by using CHX, as protein translation inhibitor, in both A2058 and A375 melanoma cells, suggesting that vanillin causes inhibition of de novo HIF-1α protein synthesis.