In this study, we showed that MART-10, a C-2 substituted, 19-nor analog of 1α,25(OH)2D3, possessed much more potent anti-tumor growth effect on human CCA in vitro and in vivo through induction of cell cycle arrest at G0/G1 phase and inhibition of NGAL. The gene discussed is LCN2; the disease is cholangiocarcinoma.