Our REST knockdown experiments in LNCaP cells further support the notion that REST downregulation not only induces NED of PCa cells but also drives NE differentiated PCa cells dedifferentiation to an EMT-like phenotype, as reflected by (i) increased Twist1 expression (Fig. 3B) and (ii) inhibition of cell migration by Twist1 knockdown in REST downregulation induced NE differentiated PCa cells (Fig. 3E). This evidence concerns the gene REST and posterior cortical atrophy.