RNA-seq analyses show that (i) short-term REST knockdown induces only NED, (ii) long-term REST knockdown induces EMT and stemness in NE differentiated LNCaP cells and (iii) both short- and long-term REST overexpression repress EMT and stemness of CWR22Rv1 cells, a castration-resistant PCa cell line. Here, REST is linked to posterior cortical atrophy.