TNF and Allergy: Specifically, the data demonstrate that an extensive panel of pro-inflammatory (CCL2, CXCL10, IL-18, TNFα, IL-6), anti-inflammatory (IL-10, sTNF-RII, IL-1Ra), acute phase proteins (CRP, PTX3) and other biomarkers (sST2, IL-1RAcP) linked to allergy and autoimmunity and other inflammatory diseases in basic discovery research are readily detectable, even in healthy control individuals, and that they remain stable for repeat analysis despite multiple freeze thaw cycles.