Using a panel of 14 pro-inflammatory (CCL2, CXCL10, IL-18, TNFα, IL-6), anti-inflammatory (IL-10, sTNF-RII, IL-1Ra), acute phase proteins (CRP, PTX3) and other biomarkers (sST2, IL-1RAcP) of inflammatory disorders previously linked to asthma, other atopic disorders [28–31] and a wide range of other chronic inflammatory conditions in humans, we quantify the sensitivity to initial freezing as well as to repeated F/T cycles that are inevitable if large studies incorporate analyses of immunological intermediate phenotypes. Here, PTX3 is linked to asthma.