TNF and Hepatitis: LPS/D-Gal-induced liver injury in mice is a well-established experimental hepatitis model.13 The hepatocyte damage in this model largely depends on the early production of detrimental inflammatory mediators such as tumor necrosis factor alpha (TNF-α), and these deleterious factors might induce massive hepatocyte apoptosis and lethal outcomes at the late stage.14, 15 In the present study, the phosphorylation status of AMPK was determined.