To further verify this association of the Drosha expression pattern and the malignancy of GC, the expressions and distribution of Drosha were assessed in four of the poorly differentiated GC cells (MKN-28, NUGC-3, BGC-803 and HGC-27) and the well-differentiated GC cell (NCL-87) by western blot and immunofluorescence (IF) staining; as expected, the enhanced nuclear Drosha was observed in malignant GC cells (Figures 1d and e). This evidence concerns the gene DROSHA and gastric cancer.