To investigate whether rhubarb can inhibit renal fibrosis and interfere with autophagic activity in rats with Ade-induced renal tubular injury, we examined the changes in immunohistochemical staining of the fibrotic markers collagen type I (collagen I) and fibronectin, as well as autophagic marker LC3 in the kidney. Here, FN1 is linked to renal fibrosis.