MIF protein and mRNA expression was analyzed by Western blotting, ELISA and RT-PCR in uterine cervical cancer cell lines SiHa and CaSki and their supernatant, and on 80 biopsies (cervical dysplasias,in situ carcinomas and invasive carcinomas) of uterine cervical tissue. The gene discussed is MIF; the disease is in situ carcinoma.