PCK1 and fatty liver disease: However, CR led to decreased expression of hepatic fatty acid and cholesterol synthesis genes (Fasn and Srebp1c, respectively), increased expression of the fatty acid oxidation gene Cpt1a, and increased the gluconeogenesis gene Pck1. Taken together, these results suggest that 10,12 CLA worsens hepatic steatosis by increasing triglyceride storage and inflammation in the liver, while CR improves hepatic steatosis by simultaneously reducing fatty acid synthesis and cholesterol synthesis gene programs while increasing fatty acid oxidation.