However, Akt, a downstream marker of the mTORC2 complex in the mTOR pathway, did not appear to be affected (Fig. S4I‐J), indicating that rapamycin inhibited ADPKD cyst formation via the mTORC1 pathway, which might not be through mTORC2 40, 48. Here, AKT1 is linked to autosomal dominant polycystic kidney disease.