INS and obesity due to melanocortin 4 receptor deficiency: To further explore the consequences of the HFD‐induced obesity at the PG level, we carried out a comprehensive characterization of the expression and regulation of key components of the GH, insulin and IGF1 regulatory axes in mice fed a LFD or a HFD, and compared them with the changes observed in the pituitary, an endocrine gland wherein these regulatory axes also play relevant functional roles 45, 48, 59.