To further explore the consequences of the HFD‐induced obesity at the PG level, we carried out a comprehensive characterization of the expression and regulation of key components of the GH, insulin and IGF1 regulatory axes in mice fed a LFD or a HFD, and compared them with the changes observed in the pituitary, an endocrine gland wherein these regulatory axes also play relevant functional roles 45, 48, 59. Here, IGF1 is linked to obesity due to melanocortin 4 receptor deficiency.