A previous study showed that AMPK activates autophagy by inhibiting mTOR and directly phosphorylating ULK1.25 To determine whether regulation of mTOR by NOS1 in NPC cells is dependent on AMPK activity, we used western blotting to detect the levels of p-AMPK and AMPK proteins in NPC cells treated with a NOS1 inhibitor or NO donor. The gene discussed is NOS1; the disease is nasopharyngeal carcinoma.