Experimental studies found that, after CRD stimulation, model rats presented with increased excitability of visceral responsive neurons in the RVM, with an abnormal increase in c-fos positive neurons; EA treatment markedly inhibited the expression of c-fos positive neurons in the RVM of model rats with IBS and thus reduced the abnormally high excitability of visceral responsive neurons in the RVM. This evidence concerns the gene FOS and irritable bowel syndrome.