TGFB1 and neoplasm: To investigate tumor-derived TGF-β effects in MDSC recruitment into liver and immune suppression conduction, sex- and age-matched WT mice were either untreated or inoculated with B16 cells or shRNA-transfected stable B16 cells prepared in our laboratory (26) (Figure S3A in Supplementary Material) (1 × 106 cells/mouse), which named TB (B16) or TB (sh B16), respectively (Figure S3B in Supplementary Material).