To elucidate the mechanisms underlying the regulatory effect of miR-30d on VEGFA expression in PCa cells, we combined gene expression profile, three miRNA target prediction algorithms, luciferase report assay, the correlation analysis in both PCa tissues and cell lines, with both in vitro and in vivo functional analyses, to confirm that miR-30d may exert its oncogenic role at least in part by suppressing MYPT1, which is involved into the Rho/Rho-kinase pathway [43]. Here, VEGFA is linked to posterior cortical atrophy.