As shown in Additional file 3: Figure S1a and Additional file 4: Table S3, significantly higher levels of POU5F1, DKK1, NOS2, MSL1, MYCBP, NRCAM, LEF1, and PPARD transcripts were found in HCC tissues versus adjacent non-tumorous liver tissues. The gene discussed is NOS2; the disease is hepatocellular carcinoma.