Despite all the above reported in vitro and in vivo results, which together demonstrate the inhibitory effect of LCn-3 PUFA on ERK and Akt activation, Wu et al. [91] have recently reported that the treatment of MCF-7 breast cancer cells in vitro with high doses of EPA or DHA (100 and 200 μM) attenuated apoptosis induced by the anti-estrogenic drug Tamoxifen, and induced p-ERK and p-Akt expression. The gene discussed is MAPK1; the disease is breast cancer.