The LCn-3 PUFA-induced inhibition of Akt phosphorylation was convincingly demonstrated by the same authors [89] also by using additional animal models of breast cancer: (a) nude mice implanted with fat-1 expressing MDA-MB-231 breast cancer cell lines; (b) fat-1 transgenic severe combined immune deficiency (SCID) mice implanted with breast tumor cells; and (c) fat-1 transgenic mouse mammary tumor viruspolyoma virus middle T oncogene double-hybrid mice. The gene discussed is FAT1; the disease is breast cancer.