TGFβ2 is elevated in the AH and TM of POAG patients and induces ocular hypertension in perfusion cultured human and porcine anterior segments, as well as in mouse eyes.9,18, , , , –23 In cultured TM cells, TGFβ2 induces the expression of ECM proteins such as fibronectin (FN) and factors that suppress proteolytic degradation of the ECM.24, –26 Additionally, TGFβ2 increases the expression of ECM cross-linking enzymes such as lysyl oxidase (LOX)27 and transglutaminase-2.28,29. This evidence concerns the gene FN1 and ocular hypertension.