To further evaluate the involvement of PEPCK1 sumoylation in Nur77-suppressed HCC samples, we performed xenograft tumour experiments in which PEPCK1 or PEPCK1K124R was first transfected into Huh7 cells, and then, Nur77 was stably expressed separately in these two cell lines (Supplementary Fig. 5g). Here, NR4A1 is linked to hepatocellular carcinoma.