However, in the current study, when cell lysates were incubated with a de-sumoylation inhibitor NEM, we observed two additional specimens (indicated by arrowheads) above the typical PEPCK1 band (indicated by asterisk) in the mouse HCC samples that were positive via the anti-SUMO1 antibody (Fig. 3a, left). This evidence concerns the gene SUMO1 and hepatocellular carcinoma.