As the DMF5 TCR is specific for an epitope of MART-1 when presented on HLA-A2, cytokine production of selected T cells upon encountering target antigen was analysed by measuring production of IFNγ, IL-2, IL-10, IL-17A and TNFα upon 16 h incubations with the MART-1+, HLA-A2+ melanoma cell line, Mel-624. The gene discussed is IL2; the disease is melanoma.