Simultaneously, human liver cancer cell lines Huh7.0 (TLR3 -/-) and Huh7.5.1 (TLR-/- RIG-I -/-) were infected with SFTSV (MOI = 1.0) for 2 hours and cultured for 48hours, expression RNA levels of IFNα and IFNβ were quantified by real-time PCR. The gene discussed is IFNB1; the disease is liver cancer.